Methodology

HIV-1/-2 antigen and antibody screen by the chemiluminescence immunoassay method (Siemens 4th Gen).  

If HIV-1/-2 screen result is reactive, then HIV-1/-2 antibody differentiation test (by membrane immunoconcentration method) is referred to Mayo Medical Laboratories.  Depending on the HIV-1 and HIV-2 antibody results of this differentiation test, subsequent reflex tests such as HIV-1 antibody confirmation by Western blot and HIV-2 antibody confirmation by line immunoassay will be performed when appropriate.