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Test Code CIFS Cutaneous Immunofluorescence Antibodies, IgG and IgG4, Serum

Additional Codes

EPIC:  LAB14078

SUNQUEST:  CIFSM

Reporting Name

Cutaneous Immfluor. Ab, IgG/IgG4, S

Useful For

Confirming the presence of IgG and/or IgG4 antibodies to diagnose pemphigoid, pemphigus, epidermolysis bullosa acquisita, or bullous lupus erythematosus

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
CIFST CIFS Titer, IgG, S No No

Testing Algorithm

If IgG anti-cell surface or anti-basement membrane zone antibodies are present, then the IgG antibody titer will be reflexed and performed at an additional charge.

Method Name

Indirect Immunofluorescence Assay (IFA)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Negative in normal individuals

Day(s) Performed

Monday through Friday

CPT Code Information

88346

88350

88350 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CIFS Cutaneous Immfluor. Ab, IgG/IgG4, S In Process

 

Result ID Test Result Name Result LOINC Value
21539 Cell Surface Ab IgG 93233-5
21540 Basement Membrane IgG 29994-1
21542 Primate Split Skin IgG 104832-1
622319 Cell Surface Ab IgG4 In Process
622320 Basement Membrane IgG4 In Process
622322 Primate Split Skin IgG4 In Process
21638 Other 48767-8

Clinical Information

Immunoglobulin G (IgG) and/or IgG4 anti-basement membrane zone (BMZ) antibodies are produced by patients with pemphigoid, pemphigus, and other rare autoimmune blistering disorders such as epidermolysis bullosa acquisita and bullous lupus erythematosus. In most patients with bullous pemphigoid, serum contains IgG anti-BMZ antibodies, while in cicatricial pemphigoid circulating IgG anti-BMZ antibodies are found in a minority of cases.

 

Circulating IgG4 is also variably present. Sensitivity of detection of anti-BMZ antibodies is increased when serum is tested using both sodium chloride-split primate skin and primate esophagus as substrates and using both IgG and IgG4 reactants.

Specimen Retention Time

30 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Report Available

2 to 7 days