Specimen Requirements

Cervical/endocervical sampling

Liquid-based cytology samples should be obtained by using either a broom-like device (Papette®) or a combination of a plastic spatula (Pap Perfect® plastic spatula) plus an endocervical sampling device (Cytobrush® Plus GT).

To obtain an adequate sampling from the cervix using a Broom-Like Device insert the central bristles of the broom into the endocervical canal deep enough to allow the shorter bristles to fully contact the ectocervix. Push gently, and rotate the broom in a clockwise direction five times. Rinse the broom as quickly as possible into the PreservCyt Solution vial by pushing the broom into the bottom of the vial 10 times, forcing the bristles apart. Swirl the broom vigorously to further release material. Discard the collection device. Tighten the cap so that the torque line on the cap passes the torque line on the vial. Label the specimen and send to the lab with appropriately completed requisition.

To obtain an adequate sample using the Endocervical Brush/Spatula Device, obtain an adequate sampling from the ectocervix using a plastic spatula. Rinse the spatula as quickly as possible into the PreservCyt Solution vial by swirling the spatula vigorously in the vial 10 times. Discard the spatula. Obtain an adequate sampling from the endocervix using an endocervical brush device. Insert the brush into the cervix until only the bottom-most fibers are exposed. Slowly rotate ¼ or ½ turn in one direction (do not over-rotate). Rinse the brush as quickly as possible in the PreservCyt Solution by rotating the device in the solution 10 times while pushing against the PreservCyt vial 10

wall. Swirl vigorously to further release material. Discard the brush. Once the specimen has been collected and the collection device rinsed, tighten the cap so that the torque line on the cap passes the torque line on the vial.